• Desarrollo de Medicamentos de Terapias Avanzadas bajo Normas de Correcta Fabricación (NCF: EUDRALEX Parte IV del 22-11-2017) para su aplicación en la clínica.
• Desarrollo de modelos experimentales, potencialmente traslacionales, basados en la Ingeniería Tisular.
• Desarrollo de nuevos biomateriales susceptibles de ser utilizados como soportes celulares en Ingeniería Tisular.

Plataforma de Terapias Avanzadas-Sala Blanca

Planta N-1

Edificio de la Fundación para la Investigación y la Innovación Biosanitaria del Principado de Asturias (FINBA)

Avenida de Roma s/n, 33011

Oviedo (España)

Contacto:

Teléfono: 985108000 /Ext. 37570 (Laboratorio)    Ext. 72631 (Busca)

E-mail: utcmr.huca@gmail.com

El laboratorio está dotado de los siguientes equipos:

• Biorreactor con control de Tª, % CO2 y % O2
• Estufas: 37 °C y 70 °C
• Microtomo
• Baño termorregulado
• Campana extractora de gases
• Centrífugas
• Incubadores celulares
• Neveras combi
• Congelador de - 20 °C
• Congelador de - 80 °C
• Tanque de nitrógeno líquido
• Cabinas de flujo laminar
• Microscopio invertido con contraste de fases y microscopio convencional, ambos con fluorescencia y conectados a un sistema informático de análisis de imagen
• Microscopio invertido con contraste de fases y pletina termorregulada
• Lupa bifocal con luz fría
• PH metro
• PCR (convencional y a tiempo real)
• Nanodrop
• Lector de ELISA
• Equipo de Western Blot
• Thermomixer

La Sala Blanca cuenta con:

• Aislador
• Cabinas de flujo laminar de bioseguridad tipo II
• Incubador para cultivo celular bajo normas de correcta fabricación
• Centrífuga
• Contadores de partículas aéreas viables
• Contadores de partículas aéreas no viables
• SAS para paso controlado de materiales
• Estufas
• Microscopio convencional
• PHmetro
• Nevera combi
• Congelador de -80 ºC

• Allain JP, Mihaljevic I, Gonzalez-Fraile MI, Gubbe K, Holm-Harritshøj L, Garcia JM, Brojer E, Erikstrup C, Saniewski M, Wernish L, Bianco L, Ullum H, Candotti D, Lelie N, Gerlich WH, Chudy M. Infectivity of blood products from donors with occult hepatitis B virus infection. Transfusion. 2013; 53(7):1405-1415. JCR: 3,386 (Q2).
• Alvarez-Viejo M, Y Menendez-Menendez Y, Blanco-Gelaz MA , Ferrero-Gutierrez A, Fernandez- Rodríguez MA, Gala J, Otero-Hernandez J. Quantifying mesenchymal stem cells in the mononuclear cell fraction of bone marrow samples obtained for cell therapy. Transplant Proc. 2013; 45(1):434-439. JCR: 0,908 (Q4)
• Ferrero-Gutierrez A, Menendez-Menendez Y, Alvarez-Viejo M, Meana A, Escudero-Augusto D, Otero- Hernandez J. New human serum-derived albumin scaffold seeded with adipose-derived stem cells and olfactory ensheathing cells used to treat spinal cord injured rats. Histol Histopathol. 2013; 28:89-100. JCR: 2,025 (Q2).
• Rosado P, Lequerica-fernández P, Villalaín L, Peña I, Sanchez-Lasheras F, de Vicente JC. Survival model in oral squamous cell carcinoma based on clinicopathological parameters, molecular markers and support vector machines. Expert Syst Appl. 2013; 40(12):4770–4776. JCR: 3,981 (Q1).
• Fernandez-Bances I, Perez-Basterrechea M, Perez-Lopez S, Nuñez Batalla D, Fernandez Rodriguez MA, Alvarez-Viejo M, Ferrero-Gutierrez A, Menendez-Menendez Y, Garcia-Gala JM, Escudero D, Paz Aparicio J, Carnero Lopez S, Lopez Fernandez P, Gonzalez Suarez D, Otero Hernandez J. Repair of long-bone pseudoarthrosis with autologous bone marrow mononuclear cells combined with allogenic bone graft. Cytotherapy. 2013; 15(5):571-577. JCR: 3,203 (Q2).
• Gallego L, Santamarta TR, Blanco V, García-Consuegra L, Cutilli T, Junquera L. Inflammatory Myofibroblastic Tumor Of The Lung And The Maxillary Region: A Benign Lesion With Aggressive Behavior. Case Rep Dent. 2013; 2013:879792. JCR: No disponible.
• Cutilli T, Bourelaki T, Scarsella S, Fabio DD, Pontecorvi E, Cargini P, Junquera L. Pathological (late) fractures of the mandibular angle after lower third molar removal: a case series. J Med Case Rep. 2013; 30(7):121. JCR: No disponible.
• Perez-Basterrechea M, Obaya AJ, Meana A, Otero J, Esteban MM. Cooperation by fibroblasts and bone marrow-mesenchymal stem cells to improve pancreatic rat-to-mouse islet xenotransplantation. PLoS One. 2013; 8(8):e73526. JCR: 2,806 (Q1).
• Suárez-Pinilla M, Fernández-Rodríguez Á, Benavente-Fernández L, Calleja-Puerta S. Vitamin K antagonist-associated intracerebral hemorrhage: lessons from a devastating disease in the dawn of the new oral anticoagulants. J Stroke Cerebrovasc Dis. 2014; 23(4):732-742. JCR: 1,517 (Q4).
• Pelaz A, Junquera L, Gallego L, García-Consuegra L, Junquera S, Gómez C. Alternative Treatments For Oral Bisphosphonate-Related Osteonecrosis Of The Jaws: A Pilot Study Comparing Fibrin Rich In Growth Factors And Teriparatide. Med Oral Patol Oral Cir Bucal. 2014; 19(4):e320-e326. JCR: 1,156 (Q3).
• Llames S, Recuero I, Romance A, García E, Peña I, Del Valle AF, Meana A, Larcher F, Del Río M. Tissue-Engineered Oral Mucosa for Mucosal Reconstruction in a Pediatric Patient With Hemifacial Microsomia and Ankyloglossia. Cleft Palate Craniofac J. 2014; 51(2):246-251. JCR: 1,05 (Q3).
• Rosado P, Fuente E, Gallego L, Calvo N. Primary Tuberculosis Of The Palate. BMJ Case Rep. 2014; 2014. JCR: No disponible.
• Fernández Rodríguez A, García García M, Ortega de Heredia D, Herrero Puente P. Erythrocyte distribution width as a short-term prognostic factor in patients with acute heart insufficiency attended at hospital-based emergency departments. Med Clin (Barc). 2014; 143(5):235-236. JCR: 1,125 (Q3). JCR: 1,125 (Q3)
• Junquera L, Pelaz A, Gallego L, García-Consuegra L, Costilla S. Bisphosphonate-Related Osteonecrosis Associated With Dental Implants: A Microcomputed Tomography Study. Implant Dent. 2014; 23(3):258- 263. JCR: 1,107 (Q3).
• Hernando J, Villarreal P, Alvarez-Marcos F, Gallego L, García-Consuegra L, Junquera L. Comparison Of Related Complications: Sentinel Node Biopsy Versus Elective Neck Dissection. Int J Oral Maxillofac Surg. 2014; 23(3):258-263. JCR: 1,918 (Q2).
• Gallego L, Junquera L, Pelaz A, García-Consuegra L, Alvarez-Arenal A, Costilla S. Sinus Mucosal Thickening In Bisphosphonate-Related Osteonecrosis Of The Jaws: A Case-Control Study. ORL J Otorhinolaryngol Relat Spec. 2014; 76(4):199-206. JCR: No disponible.
• Carrero M, Junquera L, de Vicente JC, Fresno MF. Extension of calcifyint epithelial odontogenic tumor of the maxillary sinus: a case report. Open Journal of Stomatology. 2014; 4:280-284. JCR: No disponible
• Ascani G, Campisi G, Junquera Gutierrez LM. Current controversies in classification, management, and prevention of bisphosphonate-related osteonecrosis of the jaw. Int J Dent. 2014; PMID: 25587277. JCR: No disponible.
• Pelaz A, Junquera L, Gallego L, García-Consuegra L, García-Martínez L, Cutilli T, Olay S. Epidemiology, Pharmacology And Clinical Characterization Of Bisphosphonate-Related Osteonecrosis Of The Jaw. A Retrospective Study of 70 Cases. Acta Otorrinolaringol Esp. 2015; 66(3):139-147. JCR: No disponible.
• Alvarez-Viejo M, Menendez-Menendez Y, Otero-Hernandez J. CD271 as a marker to Identify Mesenchymal Stem Cells from Diverse Sources before Culture. World J Stem Cells. 2015; 7(2):470-476. JCR: No disponible.
• Escudero D, Otero J, Perez-Basterrechea M, Vega JA, Murias E, Martin L, Forcelledo L, Lopez-Amor L, Gonzalo JA, del Busto C, Vega P. Hyperthermia in brain dead patients. Anaesth Intensive Care. 2015; 43(2):269-270. JCR: 1,695 (Q3).
• E Correia Alvarez, E Gomez Piñeiro, D Martin, S Carrocera, S Perez Lopez, N Peynot, C Giraud-Delville, JN Caamaño, A Balseiro, O Sandra, V Duranthon, M Muñoz. Early embryonic and endometrial regulation of tumor necrosis factor and tumor necrosis factor receptor 2 in the cattle uterus. Theriogenology. 2015; 83(6):1028-1037. JCR: 1,986 (Q1).
• Escudero D, Valentín MO, Escalante JL, Sanmartín A, Perez-Basterrechea M, de Gea J, Martín M, Velasco J, Pont T, Masnou N, de la Calle B, Marcelo B, Lebrón M, Pérez JM, Burgos M, Gimeno R, Kot P, Yus S, Sancho I, Zabalegui A, Arroyo M, Miñambres E, Elizalde J, Montejo JC, Domínguez-Gil B, Matesanz R. Intensive care practices in brain death diagnosis and organ donation. Anaesthesia. 2015; 70(10):1130-1139. JCR: 4,741 (Q1).
• Schilling C, Stoeckli SJ, Haerle SK, Broglie MA, Huber GF, Sorensen JA, BakholdtV, Krogdahl A, von Buchwald C, Bilde A, Sebbesen LR, Odell E, Gurney B,O'Doherty M, de Bree R, Bloemena E, Flach GB, Villarreal PM, Fresno Forcelledo MF, Junquera Gutiérrez LM, Amézaga JA, Barbier L, Santamaría-Zuazua J, Moreira A, Jacome M, Vigili MG, Rahimi S, Tartaglione G, Lawson G, Nollevaux MC, Grandi C, Donner D, Bragantini E, Dequanter D, Lothaire P, Poli T, Silini EM, Sesenna E, Dolivet G, Mastronicola R, Leroux A, Sassoon I, Sloan P, McGurk M.«Sentinel european node trial (sent): 3-year results of sentinel node biopsy in oral cancer». Eur J Cancer. 2015; 51:2777-2784. JCR: 6,029 (Q1).
• Delgado E, Perez-Basterrechea M, Suarez-Alvarez B, Zhou H, Revuelta EM, Garcia-Gala JM, Perez S, Alvarez-Viejo M, Menendez E, Lopez-Larrea C, Tang R, Zhu Z, Hu W, Moss T, Guindi E, Otero J, Zhao Y. Modulation of Autoimmune T-Cell Memory by Stem Cell Educator Therapy: Phase 1/2 Clinical Trial. EBioMedicine. 2015; 2(12):2024-2036. JCR: 6,183 (Q1).
• Correia Alvarez, E. Gomez Piñeiro, D. Martin, S. Carrocera, S. Perez, J.Otero, N. Peynot, C. Giraud- Delville, J.N.Caamaño, O. Sandra, V. Duranthon, M. Muñoz. Expression and localization of interleukin 1 beta and interleukin 1 receptor (type I) in the bovine endometrium and embryo. J Reprod Immunol. 2015; 110:1-13. JCR: 2,798 (Q2).
• González-López TJ, Pascual C, Álvarez-Román MT, Fernández-Fuertes F, Sánchez-González B, Caparrós I, Jarque I, Mingot-Castellano ME, Hernández-Rivas JA, Martín-Salces M, Solán L, Beneit P, Jiménez R, Bernat S, Andrade MM, Cortés M, Cortti MJ, Pérez-Crespo S, Gómez-Núñez M, Olivera PE, Pérez-Rus G, Martínez-Robles V, Alonso R, Fernández-Rodríguez A, Arratibel MC, Perera M, Fernández- Miñano C, Fuertes-Palacio MA, Vázquez-Paganini JA, Gutierrez-Jomarrón I, Valcarce I, de Cabo E, Sainz A, Fisac R, Aguilar C, Paz Martínez-Badas M, Peñarrubia MJ, Calbacho M, de Cos C, González-Silva M, Coria E, Alonso A, Casaus A, Luaña A, Galán P, Fernández-Canal C, Garcia-Frade J, González-Porras JR. Successful discontinuation of eltrombopag after complete remission in patients with primary immune thrombocytopenia. Am J Hematol. 2015; 90(3):E40-E43. JCR: 5,275 (Q1).
• de Vicente JC, Rodríguez-Santamarta T, Peña I, Villalaín L, Fernández-Valle Á, González-García M. Relevance of level IIb neck dissection in oral squamous cell carcinoma. Med Oral Patol Oral Cir Bucal. 2015; 20(5):e547-e553. JCR: 1,156 (Q3).
• Gallego L, Pérez-Basterrechea M, García-Consuegra L, Álvarez-Viejo M, Megías J, Novoa A, Costilla S, Meana Á, Junquera L. Repair of segmental mandibular bone defects in sheep using bone marrow stromal cells and autologous serum scaffold: a pilot study. J Clin Periodontol. 2015; 42(12):1143-1151. JCR: 3,477 (Q1).
• Contreras E, de la Rubia J, Del Río-Garma J, Díaz-Ricart M, García-Gala JM, Lozano M; Grupo Español de Aféresis. Diagnostic and therapeutic guidelines of thrombotic microangiopathies of the Spanish Apheresis Group. Med Clin (Barc). 2015; 144(7):331.e1-331.e13. JCR: 1,125 (Q3)
• García-Dasí M, Aznar JA, Jiménez-Yuste V, Altisent C, Bonanad S, Mingot E, Lucía F, Giménez F, López MF, Marco P, Pérez R, Fernández MÁ, Paloma MJ, Galmes B, Herrero S, García-Talavera JA. Adherence to prophylaxis and quality of life in children and adolescents with severe haemophilia A. Haemophilia. 2015; 21(4):458-464. JCR: 3.569 (Q2).
• Hernando J, Villarreal P, Álvarez-Marcos F, García-Consuegra L, Gallego L, Junquera L. Sentinel Node Biopsy Versus Elective Neck Dissection. Which Is More Cost-Effective? A Prospective Observational Study. J Craniomaxillofac Surg. 2016; 44(5):550-556. JCR: 1,583 (Q2).
• González-López TJ, Alvarez-Román MT, Pascual C, Sánchez-González B, Fernández-Fuentes F, Jarque I, Pérez-Rus G, Pérez-Crespo S, Bernat S, Hernández-Rivas JA, Andrade MM, Cortés M, Gómez-Nuñez M, Olivera P, Martínez-Robles V, Fernández-Rodríguez A, Fuertes-Palacio MA, Fernández-Miñano C, de Cabo E, Fisac R, Aguilar C, Bárez A, Peñarrubia MJ, García-Frade LJ, González-Porras JR. Eltrombopag safety and efficacy for primary chronic immune thrombocytopenia in clinical practice. Eur J Haematol. 2016; 97(3):297-302. JCR: 2,653 (Q2).
• Peña González I, Álvarez-Viejo M, Alonso-Montes C, Menéndez-Menéndez Y, Gutiérrez Álvarez F, de Vicente Rodríguez JC, Otero Hernández J, Meana Infiesta A. Regeneration of mandibular defects using adipose tissue mesenchymal stromal cells in combination with human serum-derived scaffolds. J Craniomaxillofac Surg. 2016; 44(9):1356–1365. JCR: 1,583 (Q2).  
• De Vicente JC, Peña I, Braña P, Hernández-Vallejo G. The use of piezoelectric surgery to lateralize the inferior alveolar nerve with simultaneous implant placement and immediate buccal cortical bone repositioning: a prospective clinical study. Int J Oral Maxillofac Surg. 2016; 45(7):851–857. JCR: 1,918 (Q2).
• Robla Costales D, Junquera L, García Pérez E, Gómez Llames S, Álvarez-Viejo M, Meana-Infiesta A. Ectopic bone formation during tissue-engineered cartilage repair using autologous chondrocytes and novel plasma-derived albumin scaffolds. J Craniomaxillofac Surg. 2016; 44(10):1743-174. JCR: 1,583 (Q2).
• Feito J, Ramos-García JL, Gago Á, Cobo JL, García-Suárez O, Junquera LM, Vega JA. Pacinian corpuscles in a cervical chondrocutaneous remnant: a case report and update of pacinian corpuscles. Am J Dermatopathol. 2016; 38:231-235. JCR: 1,095 (Q4).
• Tartaglione G, Stoeckli SJ, de Bree R, Schilling C, Flach GB, Bakholdt V, Sorensen JA, Bilde A, von Buchwald C, Lawson G, Dequanter D, Villarreal PM, Forcelledo MF, Amezaga JA, Moreira A, Poli T, Grandi C, Vigili MG, O'Doherty M, Donner D, Bloemena E, Rahimi S, Gurney B, Haerle SK, Broglie MA, Huber GF, Krogdah AL, Sebbesen LR, Odell E, Junquera Gutierrez LM, Barbier L, Santamara-Zuazua J, Jacome M, Nollevaux MC, Bragantini E, Lothaire P, Silini EM, Sesenna E,Dolivet G, Mastronicola R, Leroux A, Sassoon I, Sloan P, Colletti PM, Rubello D, McGurk M. Sentinel node in oral cancer: the nuclear medicine aspects. A survey from the sentinel european node trial. Clin Nucl Med. 2016; 41(7):534-542. JCR: 4,563 (Q1).
• Escudero D, Otero J, Menéndez de León B, Perez-Basterrechea M. Organ Donation and Elective Ventilation: A Necessary Strategy. BioMed Res Int. 2017; 2017:7518375. JCR: 2,476 (Q2).
• Fontanil T, Álvarez-Teijeiro S, Villaronga MÁ, Mohamedi Y, Solares L, Moncada-Pazos A, Vega JA, García-Suárez O, Pérez-Basterrechea M, García-Pedrero JM, Obaya AJ, Cal S. Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells. Oncotarget. 2017; 8(8):13716-13729. JCR: 5,168 (Q1)
• Morán J, Perez-Basterrechea M, Garrido P, Díaz E, Alonso A, Otero J, Colado E, González C. Effects of Estrogen and Phytoestrogen Treatment on an In Vitro Model of Recurrent Stroke on HT22 Neuronal Cell Line. Cell Mol Neurobiol. 2017; 37(3):405-416. JCR: 2,939 (Q2).
•  Menéndez-Menéndez Y, Otero-Hernández J, Vega JA, Pérez-Basterrechea M, Pérez-López S, Álvarez-Viejo M, Ferrero-Gutiérrez A. The role of bone marrow mononuclear cell-conditioned medium in the proliferation and migration of human dermal fibroblasts. Cell Mol Biol Lett. 2017; 22:29-40. JCR: 1,26(Q4)
• Zhao Y, Jiang Z, Delgado E, Li H, Zhou H, Hu W, Perez-Basterrechea M, Janostakova A, Tan Q, Wang J, Mao M, Yin Z, Zhang Y, Li Y, Li Q, Zhou J, Li Y, Martinez Revuelta E, Maria García-Gala J, Wang H, Perez-Lopez S, Alvarez-Viejo M, Menendez E, Moss T, Guindi E, Otero J. Platelet-Derived Mitochondria Display Embryonic Stem Cell Markers and Improve Pancreatic Islet β-cell Function in Humans. Stem Cells Transl Med. 2017; 6(8):1684-1697. JCR: 4 (Q1)
• Perez-Basterrechea M, Esteban MM, Alvarez-Viejo M, Fontanil T, Cal S, Sanchez Pitiot M, Otero J, Obaya AJ. Fibroblasts accelerate islet revascularization and improve long-term graft survival in a mouse model of subcutaneous islet transplantation. PLoS One. 2017; 12(7):e0180695. JCR: 2,806 (Q1)
• Perez-Basterrechea M, Esteban MM, Vega JA, Obaya AJ. Tissue engineering approaches in pancreatic islet transplantation. Biotechnol Bioeng. 2018 (Epub ahead of print). JCR: 4,481 (Q1)