Platelets: Biology, Pathology and Therapeutic Applications

Basic Research

Figure-Grupo-Lab-jan23

Associated Clinical Staff

Figure-Grupo-v4-mar-22

The Platelets research group’s origins date back to 2010, when Laura Gutiérrez became head of her own research group as Junior Group Leader at Sanquin Research (Amsterdam, the Netherlands). At Sanquin Research, she supervised two PhD researchers, a senior laboratory technician and two postdoctoral researchers, publishing in specialised, high-impact scientific journals. In August 2015, she returned to Spain on a Ramón y Cajal contract, initially in the Instituto de Investigación Sanitaria San Carlos, where she supervised a postdoctoral researcher, a laboratory technician and students from the Spanish medical residency programme, who contributed to the group’s research projects and the production of thematic reviews. In January 2018, she moved to ISPA, establishing this research group by recruiting highly-respected associated clinical researchers and gradually incorporating PhD researchers and a postdoctoral researcher.

The work of the Platelets research group focuses on megakaryocytic differentiation, and the production and function of platelets in health and illness. Specifically, we are interested in the mechanisms of transcriptional regulation and protein translation during megakaryopoiesis: how they can be affected by haematological and non-haematological pathologies of different types and, in turn, affect platelet quality and function. We are particularly interested in megakaryocytic and haematopoietic differentiation, and how different conditions of stress or pathological situations influence this process. By understanding how haematopoiesis is regulated normally or in conditions of stress, we can use this knowledge to help develop new and/or personalised therapies. We take a multidisciplinary approach to research, and have therefore established collaborations with clinical departments such as psychiatry, ophthalmology, cardiovascular medicine, dermatology, paediatrics, etc. Through these collaborations, we intend to study the function or role or platelets in non-haematological pathologies, and also explore the use of platelets for diagnosis, prognosis and the development of platelet-based therapeutic applications.

• Studying the physiopathology of congenital, acquired or autoimmune thrombocytopenia, and thrombocytopenia in other hematological pathologies.
• Studying the function and molecular characteristics of platelets in Hemophilic patients.
• Studying the production and function of platelets in non-hematological pathologies.
• Studying the function of platelets during embryo development, in newborns and infants.
• Studying the function of platelets under anesthesia (and its management).
• Development of new platelet-based therapies.

• AUTHORS: Martínez-Botía P. Villar P., Carbajo-Argüelles G., Jaiteh Z., Acebes-Huerta A. and Gutiérrez L.
  TITLE: Proteomics-wise, how similar are mouse and human platelets?
  Platelets, 2023. In Press.
  IF 3.378; Q1
• AUTHORS:  Paniagua G., González-Blanco L., Sáiz P.A., Moya-Lacasa C., Gutiérrez L., Martínez-Botía P., Dal Santo F., Jiménez-Treviño L., García-Portilla M.P., Bobes J.
  TITLE: Platelet and white blood-cell-based ratios: Differential inflammatory markers of severe mental disorders?
  Revista de Psiquiatría y Salud Mental, 2023. In Press.
  IF 6.795; Q2
• AUTHORS: Bernardo Á, Caro A, Martínez-Carballeira D, Corte JR, Vázquez S, Palomo-Antequera C, Andreu A, Fernández-Pardo Á, Oto J, Gutiérrez L*, Soto I* and Medina P*.
  TITLE: Applicability of the Thrombin Generation Test to Evaluate the Hemostatic Status of Hemophilia A Patients in Daily Clinical Practice.
  Journal of Clinical Medicine, 2022. Volume 11, issue 12, 3345. * equal contribution.
  IF 4.964; Q1 (Open access)
• AUTHORS: Martínez-Botía P, Meinders M, De Cuyper IM, Eble JA, Semple JW, Gutiérrez L.
  TITLE: Platelet Proteomics to Understand the Pathophysiology of Immune Thrombocytopenia: Studies in Mouse Models.
  Blood Adv, 2022. Volume 6, issue 11, 3529-3534.
  IF 7.637; Q1 (Open access)
• AUTHORS: Fernández-Fuertes J, Arias-Fernández T, Acebes-Huerta A, Álvarez-Rico M, Gutiérrez L.
  TITLE: Clinical Response After Treatment of Knee Osteoarthritis with a Standardized, Closed-System, Low-Cost Platelet-Rich Plasma Product: 1-Year Outcomes.
  Orthop J Sports Med, 2022. Volume 10, issue 3, 23259671221076496.
  IF 2.727; Q1 (Open access)
• AUTHORS: Villa-Fajardo M, Palma MCY, Acebes-Huerta A, Martínez-Botía P, Meinders M, Nolte MA, Cuesta CB, Eble JA, Del Castillo JG, Martín-Sánchez FJ, Gutiérrez L.
  TITLE: Platelet number and function alterations in preclinical models of sterile inflammation and sepsis patients: implications in the pathophysiology and treatment of inflammation.
  Transfus Apher Sci, 2022. Volume 61, issue 2, 103413.
  IF 1.764; Q3
• AUTHORS: Acebes-Huerta A., Martínez-Botía P., Martín Martín C., Bernardo Á., Rodríguez A.R., Vicente-Ayuso M.C., Benavente Cuesta C., Gutiérrez L.
  TITLE: Immunophenotyping and cell sorting of human MKs from human primary sources or differentiated in vitro from hematopoietic progenitors.
  Journal of Visualized Experiments, 2021. Volume 174, e62569.
  IF 1.355; Q2
• AUTHORS: Lozano M.L., Segú-Vergés C., Coma M., Álvarez-Roman M.T., González-Porras J.R., Gutiérrez L., Valcárcel D. and Butta N.
  TITLE: Elucidating the Mechanism of Action of the attributed Immunomodulatory Role of Eltrombopag in Primary Immune Thrombocytopenia: an In Silico Approach.
  International Journal of Molecular Sciences, 2021. Volume 22, issue 13, e6907.
  IF 6.208; Q1 (Open access)
• AUTHORS: Martínez-Botía P.,* Bernardo Á.,* Acebes-Huerta A.,* Caro A., Leoz B., Martínez-Carballeira D., Palomo-Antequera C., Soto I.,^$ and Gutiérrez L.^$
  TITLE: Clinical management of hypertension, inflammation and thrombosis in hospitalized COVID-19 patients: impact on survival and concerns.
  Journal of Clinical Medicine, 2021. Volume 10, issue 5, 1073. *,^$ equal contribution.
  IF 4.964; Q1 (Open access)
• AUTHORS: Scheenstra M.R.,* Martínez-Botía P.,* Acebes-Huerta A., Brouwer R.W.W., Caballero-Sánchez N., Gillemans N., de Bleser P., Nota B., De Cuyper I.M., Salunkhe V., Woltman A.M., van de Laar L., Rijkers E., Demmers J.A.A., van IJcken W.F.J., Philipsen S., van den Berg T.K., Kuijpers T.W. and Gutiérrez L.
  TITLE: Comparison of the PU.1 transcriptional regulome and interactome in human and mouse inflammatory dendritic cells.
  Journal of Leukocyte Biology, 2021. Volume 110, issue 4, 735-751. *equal contribution.
  IF 4.962; Q1
• AUTHORS: Martínez-Botía P., Acebes-Huerta A., Seghatchian J., Gutiérrez L.
  TITLE: On the quest for in vitro platelet production by re-tailoring the concepts of megakaryocyte differentiation.
  Medicina, 2020. Volume 56, issue 12, 671.
  IF 1.467; Q3 (Open access)
• AUTHORS: Papadopoulos P., Kafasi A., De Cuyper I.M., Barroca V., Lewandowski D., Kadri Z., Veldthuis M., Berghuis J., Gillemans N., González A., Grosveld F.G., van Zwieten R., Philipsen S., Vernet M.*, Gutiérrez L.* and Patrinos G.P.*
  TITLE: Mild dyserythropoiesis and b-like globin gene expression imbalance due to the loss of histone chaperone ASF1B.
  Human Genomics, 2020. Volume 14, issue 1, 39. *equal contribution.
  IF 4.639; Q1 (Open access)
• AUTHORS: Martínez-Botía P., Acebes-Huerta A., Seghatchian J.*, Gutiérrez L.*
  TITLE: In vitro platelet production for transfusion purposes: Where are we now?
  Transfus Apher Sci, 2020. Volume 59, issue 4, 102864. *equal contribution.
  IF 1.764; Q3
• AUTHORS: Martínez-Botía P., Velasco A., Rolle V., Jiménez-Trevino L., de la Fuente-Tomás L., Bernardo A., Arias T., Muñoz-Turrillas M.C., García-Alvarez L., González-Blanco L., Rodríguez-Revuelta J., García-Portilla M.P., Courtet P., Bobes J., Gutiérrez L.,* Sáiz P.A.*
  TITLE: Sex-dependent grades of haematopoietic modulation in patients with major depressive disorder episodes are associated with suicide attempts.
  European Neuropsychopharmacology, 2020. Volume 40, 17-30. *equal contribution.
  IF 4.600; Q1; (Open access)
• AUTHORS: Hansen M., Zeddies S., Meinders M., di Summa F., Rollmann E., van Alphen F.P.J., Hoogendijk A.J., Moore K.S., Halbach M., Gutiérrez L., van den Biggelaar M., Thijssen-Timmer D.C., Auburger G.W.J., van den Akker E., von Lindern M.
  TITLE: The RNA-binding protein ATXN2 controls cellular processes during megakaryopoiesis.
  International Journal of Molecular Sciences, 2020. Volume 21, issue 3, 967.
  IF 5.542; Q1; (Free PMC)
• AUTHORS: Acebes-Huerta A., Arias-Fernández T., Bernardo A., Muñoz-Turrillas M.C., Fernández-Fuertes J., Seghatchian J., Gutiérrez L.
  TITLE: Platelet-derived bio-products: classification update, applications, concerns and new perspectives.
  Transfus Apher Sci, 2020. Volume 59, issue 1, e102716.
  IF 1.764; Q3
• AUTHORS: Velasco A., Rodríguez-Revuelta J., Oliée E., Abadd I., Fernández-Peláez A., Cazalse A., Guillaumee S., de la Fuente-Tomás L., Jiménez-Treviño L., Gutiérrez L., García-Portilla P., Bobes J., Courtete P., Sáiz P.A.
  TITLE: Neutrophil-to-lymphocyte ratio: a potential new peripheral biomarker of suicidal behaviour.
  European Psychiatry, 2020. Volume 63, issue 1, e14.
  IF 5.361; Q1; (Free PMC)
• AUTHORS: Acebes-Huerta A., Arias-Fernández T., Bernardo A., Muñoz-Turrillas M.C., Fernández-Fuertes J., Seghatchian J., Gutiérrez L.
  TITLE: Platelet-derived bio-products: classification update, applications, concerns and new perspectives.
  Transfus Apher Sci, 2019. In Press.
  IF 0.963; Q4
• AUTHORS: Velasco A., Rodríguez-Revuelta J., Oliée E., Abadd I., Fernández-Peláez A., Cazalse A., Guillaumee S., de la Fuente-Tomás L., Jiménez-Treviño L., Gutiérrez L., García-Portilla P., Bobes J., Courtete P., Sáiz P.A.
  TITLE: Neutrophil-to-lymphocyte ratio: a potential new peripheral biomarker of suicidal behaviour.
  European Psychiatry, 2019, In Press.
  IF 3.941; Q1
• AUTHORS: Gutiérrez L., Caballero N., Fernández-Calleja L., Karkoulia E., Strouboulis J.
  TITLE: Regulation of GATA1 levels in erythropoiesis.
  IUBMB Life, 2020, Volume 72, issue 1, pages 89-105.
  IF 3.051; Q1
• AUTHORS: Scully D., Sfyri P., Wilkinson H.N., Acebes-Huerta A., Verpoorten S., Muñoz-Turrillas M.C., Parnell A., Patel P., Hardman M.J., Gutiérrez L. and Matsakas A.
  TITLE: Optimising platelet secretomes to deliver robust tissue-specific regeneration.
  Journal of Tissue Engineering and Regenerative Medicine, 2019. In Press.
  IF 4.089; Q1
• AUTHORS: Ojea-Pérez A.M., Acebes-Huerta A., Arias-Fernández T, Gutiérrez L.⁎ and Muñoz-Turrillas M.C.*.
  *Equal contribution
  TITLE: Implementation of a closed platelet-rich-plasma preparation method using the local blood bank infrastructure at the Principality of Asturias (Spain): Back to basic methodology and a demographics perspective after 1 year.
  Transfus Apher Sci, 2019. Volume 98, issue 5, pages 701-704.
  IF 1.755; Q4
• AUTHORS: Scully D., Sfyri P., Verpoorten S., Papadopoulos P., Muñoz-Turrillas M.C., Mitchell R., Aburima A., Patel K., Gutiérrez L., Naseem K.M. and Matsakas A.
  TITLE: Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury.
  Acta Physiologica, 2019. Volume 225, issue 3, e13207.
  IF 5.930; D1
• AUTHORS: Salunkhe V., De Cuyper I.M., Papadopoulos P., van der Meer P.F., Daal B.B., Villa-Fajardo M., de Korte D., van den Berg T.K. and Gutiérrez L.
  TITLE: A Comprehensive Proteomics Study on Platelet Concentrates: Platelet Proteome, Storage Time and Mirasol Pathogen Reduction Technology.
  Platelets, 2019. Volume 30, issue 3, pages 368-379.
  IF 2.356; Q3
• AUTHORS: Vicente-Ayuso M.C., Papadopoulos P., Villa-Fajardo M., Prieto B., Álvarez-Carmona A.M., Mateo-Morales M., Pérez-López C., Peña-Cortijo A., Polo-Zarzuela M., Sáez I., Martín M.P., Benavente-Cuesta C., González-Fernández A., Martínez-Martínez R., Zwaginga J.J., Seghatchian J. and Gutiérrez L.
  TITLE: Identification of underlying and transfusion-related platelet qualitative alterations in the hemato-oncologic patient.
  Transfus Apher Sci, 2017. Volume 56, pages 756-768.
  IF 0.963; Q4
• AUTHORS: Vicente-Ayuso M.C., García-Roa M., González-Fernández A., Álvarez-Carmona A.M., Benavente-Cuesta C., Mateo-Morales M., Pérez-López C., Peña-Cortijo A., Polo Zarzuela M., Gutiérrez L. and Martínez-Martínez R.
  TITLE: Therapy-related myeloid neoplasms as a concerning complication in acute promyelocytic leukemia: a case report.
  Hematology Reports, 2017. Volume 9, Issue 3, pages 114-117.
  IF 0.7; Q3
• AUTHORS: García-Roa M., Vicente-Ayuso M.C., Bobes A.M., Pedraza A.C., González-Fernández A., Martín M.P., Sáez I., Seghatchian J. and Gutiérrez L.
  TITLE: Red Blood Cell Storage Time and Transfusion: current practice, concerns and future perspectives.
  Blood Transfusion, 2017. Volume 15, Issue 3, pages 221-231.
  IF 1.514; Q4
• AUTHORS: Scheenstra M.R., De Cuyper I.M., Branco-Madeira F., de Bleser P., Kool M., Meinders M., Hoogenboezem M., Mul E., Wolkers M.C., Salerno F., Nota B., Saeys Y., Klarenbeek S., van IJcken W.F.J., Hammad H., Philipsen S., van den Berg T.K., Kuijpers T.W., Lambrecht B.N. and Gutiérrez L.
  TITLE: GATA1-deficient dendritic cells display impaired CCL21-dependent migration towards lymph nodes due to reduced levels of polysialic acid.
  Journal of Immunology. 2016. Volume 197, issue 11, pages 4312-4324.
  IF 4.985; Q1
• AUTHORS: Meinders M., Hoogenboezem M., Scheenstra M.R., De Cuyper I.M., Papadopoulos P., Németh T., Mócsai A., van den Berg T.K., Kuijpers T.W. and Gutiérrez L.
  TITLE: Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment.
  PLoS One, 2016. Volume 11, issue 5, e0154342.
  IF 3.057; Q1
• AUTHORS: Salunkhe V., Papadopoulos P. and Gutiérrez L.
  TITLE: Culture of Megakaryocytes from Human Peripheral Blood Mononuclear Cells.
  Bio-Protocol, 2015. Volume 5, issue 21, e1639.
  IF N/A; Q N/A
• AUTHORS: Scheenstra M.R., Salunkhe V., De Cuyper I.M., Hoogenboezem M., Li E., Kuijpers T.W., van den Berg T.K. and Gutiérrez L.
  TITLE: Characterization of hematopoietic GATA transcription factor expression in mouse and human dendritic cells.
  Blood Cells Mol Dis, 2015. Volume 55, issue 4, pages 293-303.
  IF 2.731; Q2
• AUTHORS: Papadopoulos P., Gutiérrez L., Demmers J., Scheer E., Pourfarzad F., Papageorgiou D.N., Karkoulia E., Strouboulis J., van de Werken H.J.G., van der Linden R., Vandenberghe P., Dekkers D.H.W., Philipsen S., Grosveld F. and Tora L.
  TITLE: TAF10 interacts with GATA1 transcription factor and controls mouse erythropoiesis.
  Mol Cell Biol, 2015. Volume 35, issue 12, pages 2103-2118.
  IF 4.427; Q1
• AUTHORS: Meinders M., Kulu D.I., van de Werken H.J.G., Hoogenboezem M., Janssen H., Brouwer R.W.W., van Ijcken W.F.J., Rijkers E.-J., Demmers J.A.A., Krüger I., van den Berg T.K., Suske G., Philipsen S.* and Gutiérrez L.*
  TITLE: Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation, and platelet formation and function.
  Blood, 2015. Volume 125, issue 12, pages 1957-1967. *equal contribution.
  IF 11.841; D1
• AUTHORS: Salunkhe V., van der Meer P.F., de Korte D., Seghatchian J.* and Gutiérrez L.*
  TITLE: Development of blood transfusion product pathogen reduction treatments: a review of methods, current applications and demands.
  Transfus Apher Sci, 2015. Volume 52, issue 1, pages 19-34. *equal contribution.
  IF 0.963; Q4
• AUTHORS: Zeddies S., De Cuyper I.M., van der Meer P.F., Daal B., de Korte D., Thijssen-Timmer D.C.* and Gutiérrez L.*
  TITLE: Pathogen reduction treatment using riboflavin and ultraviolet light impairs platelet reactivity towards specific agonists in vitro.
  Transfusion, 2014. Volume 54, issue 9, pages 2292-2300. *equal contribution.
  IF 3.042; Q2
• AUTHORS: Bergink S., Theil A., Toussaint W., Clapes T., van der Linden R., Demmers J.A., Marteijn J.A., De Cuyper I.M., van Gent T., Robin C., Philipsen S., Vermeulen W., Mitchell J.* and Gutiérrez L.*
  TITLE: Erythropoietic defect associated with reduced cell proliferation in mice lacking the 26S proteasome shuttling factor Rad23b.
  Mol Cell Biol, 2013. Volume 33, issue 19, pages 3879-3892. *equal contribution.
  IF 4.427; Q1
• AUTHORS: Fujita R., Takayama-Tsujimoto M., Satoh H., Gutiérrez L., Aburatani H., Fujii S., Sarai A., Bresnick E.H., Yamamoto M. and Motohashi H.
  TITLE: NF-E2 p45 Is Important for Establishing Normal Function of Platelets.
  Mol Cell Biol, 2013. Volume 33, issue 14, pages 2659-2670.
  IF 4.427; Q1
• AUTHORS: De Cuyper I.M., Meinders M., van de Vijver E., de Korte D., Porcelijn L., de Haas M., Eble J.A., Seeger K., Rutella S., Pagliara D., Kuijpers T.W., Verhoeven A.J., van den Berg T.K. and Gutiérrez L.
  TITLE: A novel flow cytometry-based platelet aggregation assay.
  Blood, 2013. Volume 121, issue 10, e70-80.
  IF 11.841; D1

• European Researchers’ Night (27/9/2019)